Description
Title: Using the G-Quadruplex/Thioflavin T Complex as a Label-Free Fluorescence Aptasensor to Detect Trypsin
Abstract: Using thioflavin T as a fluorescent probe, a brand-new, label-free fluorescent assay has been created to detect trypsin. Cytochrome c can be used to combine a particular DNA aptamer. The G-quadruplex portion of the DNA aptamer, which has a strong affinity for thioflavin T, is made visible by trypsin hydrolysis of the cytochrome c into small peptide fragments. This increases the fluorescence’s intensity. The DNA aptamer and cytochrome c together prevented thioflavin T from binding, which reduced the fluorescence intensity when trypsin was absent. With a detection limit of 0.2 g/mL, the fluorescent biosensor displayed a linear relationship from 0.2 to 60 g/mL. Additionally, the suggested technique was successfully used to find trypsin in biological samples. This technique has a lot of potential for the quick, cheap, and selective detection of trypsin in biological and bioanalytical samples and for making accurate medical diagnoses.
Keywords: thioflavin T; trypsin; cytochrome c; DNA aptamer; label-free
Paper Quality: SCOPUS / Web of Science Level Research Paper
Subject: Chemistry
Writer Experience: 20+ Years
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