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Title: The genetic, chemical, and morphological characteristics of the native Korean thyme Bak-Ri-Hyang (Thymus quinquecostatus Celak.)
Abstract: In Korea, bak-ri-hyang (Thymus quinquecostatus Celak.) is a significant aromatic and medicinal plant. During cultivation and marketing, T. quinquecostatus is always blended with other thyme cultivars. Thus, in order to distinguish Bak-ri-hyang from other thyme cultivars, this study sought to ascertain the genetic variability and essential oil composition of three Korean native thyme cultivars, T. quinquecostatus, gathered from the Wolchul, Jiri, and Odae mountains. Gas chromatography-mass spectrometry was used to determine the composition of essential oils collected from nine different people (GC–MS). Using 16 different primers, the random amplified polymorphic DNA (RAPD) analysis was completed. According to the results of the GC-MS analysis, the cultivars Wolchul, creeping, golden, and orange belong to the geraniol chemotype. The Odae, Lemon, and Silver cultivars, on the other hand, are members of the thymol chemotype. Linalool was also the component that carpet and Jiri cultivars had the highest concentration of. The results of the RAPD analysis showed that all thyme cultivars exhibited distinctive RAPD patterns that permitted their identification. 16 primers were used to generate a total of 133 bands, 124 of which were polymorphic, or 93.2% polymorphism. A RAPD marker cluster analysis revealed a distinct separation between nine thyme cultivars. The RAPD markers’ highest dissimilarity and similarity coefficients, respectively, were 0.58 and 0.98. The nine thyme cultivars could be separated into two main clusters based on the RAPD patterns. The Wolchul and Odae cultivars were grouped together with the other three Korean cultivars, but their essential oil compositions did not cluster in the same way. The results of this study indicate that RAPD analysis can be a useful tool for differentiating T. quinquecostatus from other Thymus species using markers.
Keywords: essential oil; genetic; RAPD; thyme; Thymus quinquecostatus; Thymus vulgaris
Paper Quality: SCOPUS / Web of Science Level Research Paper
Subject: Antibiotics
Writer Experience: 20+ Years
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