Research Paper on Research Paper on Evaluation of the Antimicrobial Potential of Apis dorsata Gut Isolated Bacillus Cereus Against Neisseria Gonorrhoeae in Silico and In Vitro

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Title: Evaluation of the Antimicrobial Potential of Apis dorsata Gut Isolated Bacillus Cereus Against Neisseria Gonorrhoeae in Silico and In Vitro

Abstract: Globally, antimicrobial resistance is a significant issue for public health and development. Insects, which are already acknowledged by the scientific community as a source of natural antibiotics, are one potential source of alternative antibiotics from nature due to the pathogenic bacteria Neisseria gonorrhoeae’s growing resistance to antibiotics. As a first step in the process of developing a new drug, this study sought to assess the potential of gut-associated bacteria isolated from Apis dorsata, an Asian giant honeybee, as an antibacterial against N. gonorrhoeae. Acinetobacter indicus and Bacillus cereus were two of the gut-associated bacteria associated with A. dorsata that had a perfect identity to reference bacteria from GenBank. In an in vitro antibacterial test, B. cereus cell-free culture supernatants (CFCS) demonstrated very potent antibacterial activity against N. gonorrhoeae. In contrast to ceftriaxone, cefixime, and doxycycline, molecular docking demonstrated that antimicrobial lipopeptides from B. cereus (surfactin, fengycin, and iturin A) had a comparable value of binding-free energy (BFE) with the target protein receptor for N. gonorrhoeae, namely penicillin-binding protein (PBP) 1 and PBP2. The H-bond and hydrophobic interactions are altered, but the surfactin is still stable at the active site of PBP2, according to the molecular dynamics simulation (MDS) study. Surfactin has the greatest antibacterial potential against PBP2 of N. gonorrhoeae, according to this discovery.

Keywords: lipopeptide; Apis dorsata; Bacillus cereus; Neisseria gonorrhoeae; insect gut; antimicrobial activity

Paper Quality: SCOPUS / Web of Science Level Research Paper

Subject: Antibiotics

Writer Experience: 20+ Years

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