Description
Title: HPLC-UV Analysis Using Magnetic Molecularly Imprinted Polymers to Quickly and Strictly Extraction and Detect Methotrexatein Serum
Abstract: In this study, magnetic molecularly imprinted polymers (MMIPs), new materials for selective recognition, were created. Methotrexate (MTX) was effectively adsorbable, selectively recognized, and rapidly magnetically separated from the patient plasma using the recognition materials as pretreatment materials for magnetic molecularly imprinted solid-phase extraction (MSPE). A new technique for the clinical detection and monitoring of the MTX concentration was created by combining this method with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) to achieve accurate and quick detection of the plasma MTX concentration. By using the sol-gel method, the MMIPs for the selective adsorption of MTX were created. Transmission electron microscopy, Fourier transform-infrared spectrometry, X-ray diffractometry, and X-ray photoelectron spectrometry were used to characterize the materials. Static, dynamic, and selective adsorption experiments were used to assess the MMIPs’ MTX adsorption capabilities. The extraction conditions were systematically improved as a result. The imprinting factor was 9.40, the adsorption equilibrium time was 60 minutes, and the MMIPs had an MTX adsorption capacity of 39.56 mgg1. The following were the ideal extraction conditions: The loading time was 120 minutes, the leachate was 8:2 (v/v) water-methanol, the eluent was 4:1 (v/v) methanol-acetic acid, and the elution time was 60 minutes. The amount of MMIP was 100 mg. The detection limit for MTX was 12.51 ng mL-1, and its linearity ranged from 0.00005-0.25 mg mL-1. The MSPE-HPLC-UV method had excellent MTX detection accuracy, and the outcome was consistent with a drug concentration analyzer.
Keywords: methotrexate; magnetic molecularly imprinted polymers; magnetic molecularly imprinted solid-phase extraction; high-performance liquid chromatography–ultraviolet detection
Paper Quality: SCOPUS / Web of Science Level Research Paper
Subject: Chemistry
Writer Experience: 20+ Years
Plagiarism Report: Turnitin Plagiarism Report will be less than 10%
Restriction: Only one author may purchase a single paper. The paper will then indicate that it is out of stock.
What will I get after the purchase?
A turnitin plagiarism report of less than 10% in a pdf file and a full research paper in a word document.
In case you have any questions related to this research paper, please feel free to call/ WhatsApp on +919726999915
Reviews
There are no reviews yet.