Description
Title: E. coli -clamp inhibitor screening revealed that only a select few effectively inhibit Helicobacter pylori: Characterization of the Structure and Function
Abstract: The trait of interacting with different enzymes and the nature of -clamp during DNA replication to promote processivity makes it a significant drug target. The DNA replication machinery of Helicobacter pylori (H. pylori), which distinguishes it from other microorganisms, has a number of special characteristics. In the current study, we tested E. coli -clamp inhibitor molecules against H. pylori -clamp to determine whether differences in DNA replication proteins behavior account for any differences in drug response when compared to E. coli. The binding of inhibitors to H. pylori -clamp was examined using a variety of techniques, including docking, surface competition assay, complex structure determination, and antimicrobial assay. Three molecules, 5-chloroisatin, carprofen, and 3,4-difluorobenzamide, were co-crystallized with H. pylori -clamp out of the five inhibitors that were shortlisted based on docking score, and the structures show that they bind at the protein-protein interaction site as expected. In vivo studies revealed that only two molecules, 5-chloroisatin and 3,4-difluorobenzamide, had MIC values in the micro molar range that inhibited the growth of the pylori, which was superior to the inhibitory effect of the same drugs on E. coli. As a result, when evaluating these drugs for H. pylori, it may be possible to investigate the possibility of using them to create species-specific pharmacophore for the creation of new drugs for H. pylori.
Keywords: DNA replication; surface competition assay; β-clamp; E. coli inhibitors; structure; screening
Paper Quality: SCOPUS / Web of Science Level Research Paper
Subject: Antibiotics
Writer Experience: 20+ Years
Plagiarism Report: Turnitin Plagiarism Report will be less than 10%
Restriction: Only one author may purchase a single paper. The paper will then indicate that it is out of stock.
What will I get after the purchase?
A turnitin plagiarism report of less than 10% in a pdf file and a full research paper in a word document.
In case you have any questions related to this research paper, please feel free to call/ WhatsApp on +919726999915
Reviews
There are no reviews yet.